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    Dionex AminoPac PA1 氨基酸分析色譜柱

    更新時(shí)間:2019-11-12

    簡要描述:

    Dionex AminoPac PA1 氨基酸分析色譜柱
    特別適用于分析酸性和酸不穩(wěn)定性氨基酸,以及用陽離子交換分離不*的氨基酸對(duì)。

    Dionex AminoPac PA1 氨基酸分析色譜柱

    用途和特點(diǎn)
    AminoPac PA1柱用于氨基酸的快速分離。特別適用于分析酸性和酸不穩(wěn)定性氨基酸,以及用陽離子交換分離不*的氨基酸對(duì)。

     

     

    Dionex AminoPac PA1 氨基酸分析色譜柱

    訂貨信息:

    分析柱
    AminoPac PA1 Analytical Column (4 x 250 mm)037022
    保護(hù)柱
    AminoPac PA1 Guard Column (4 x 50 mm)037023

     

     

    For Specialized Amino Acid Applications with Postcolumn Derivatization

    The AminoPac® PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead™ particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.

    • Resolves phosphorylated and other amino acids by anion-exchange chromatography

    • Separates acid-labile amino acids under basic pH conditions

    • Separates strongly acidic amino acids

    • Requires OPA or ninhydrin postcolumn derivatization

    The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.

    The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not completely resolved by cation-exchange chromatography.

     

     

     

    AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization  Specifications
    DimensionsAminoPac PA1 Analytical Column:
    4 × 250 mm
    AminoPac PA1 Guard Column:
    4 × 50 mm
    Maximum operating pressure4000 psi (27.9 MPa)
    Recommended operating temperatureAmbient
    Typical operating conditions1.0 mL/min gradient
    Recommended flow rate: 1.0 mL/min
    Mobile phase compatibilityCompatible with up to 5% common organic solvents.
    Recommended eluents: hydroxide, borate, and acetate.
    Resin composition10-μm-diameter, pellicular substrate (2% cross-linked)
    agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked).
    Anion-exchange capacityN/A
    Column constructionPEEK with 10-32 threaded ferrule-style end fittings.
    All components are nonmetallic.

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